WHOLE GENOME SEQUENCING

Without extraction

Extraction included

• DNA extractions with Qiagen kits on QIAsymphony
• QC check with Victor Nivo Multimode Microplate Reader
• Library preparation and sample indexing with Illumina kits
• Full automated library preparation on Hamilton NGS STAR
• 30x genome coverage

WHOLE GENOME SEQUENCING
(WITHOUT DNA EXTRACTION)

• QC check with Qubit Flex Fluorometer
• Library preparation and sample indexing with Illumina kits
• Full automated library preparation on Hamilton NGS STAR
• 30x genome coverage

WHOLE EXOME SEQUENCING
(WITHOUT DNA EXTRACTION)

• QC check with VICTOR Nivo Multimode Microplate Reader and NanoDrop One
• Library preparation and sample indexing with Illumina kits
• Full automated library preparation on Hamilton NGS STAR
• ~ 8 Gb (100X) / Sample
• Library QC with Agilent TapeStation and Victor Nivo Multimode Microplate Reader

SHOTGUN METAGENOME SEQUENCING
(WITHOUT DNA EXTRACTION)

• QC check with VICTOR Nivo Multimode Microplate Reader and NanoDrop One
• Library preparation and sample indexing with Illumina kits
• Full automated library preparation on Hamilton NGS STAR
• ≥20 M Reads (R1 + R2 = 40 M)

WHOLE TRANSCRIPTOME SEQUENCING
(WITHOUT RNA EXTRACTION)

• QC with VICTOR Nivo Multimode Microplate Reader and Agilent TapeStation
• Library preparation and sample indexing with Illumina kits
• Full automated library preparation on Hamilton NGS STAR
• ≥50 M Reads (R1 + R2 = 100 M Reads)
• Library QC with Agilent TapeStation and Victor Nivo Multimode Microplate Reader

METABOLOMICS

• Metabolite Extraction
• LC-MS/MS analysis with Thermo Orbitrap Exploris 240 Mass Spectrometer
• Data processing with Compound Discoverer